Propagasi Cendawan Mikoriza Arbuskula (CMA) Glomus sp. dengan Kombinasi Pupuk NK, Hara Johnson, dan Kompos pada Inang Jagung Dalam Budidaya Greenhouse
DOI:
https://doi.org/10.24843/Keywords:
nutrient addition, wet sieving, spore count, AMF colonization, biofertilizerAbstract
Propagation of Arbuscular Mycorrhizal Fungi (AMF) Glomus sp. with the Combination of NK Fertilizer, Johnson Nutrients, and Compost on Maize Host in Greenhouse Cultivation. Indonesian farmers are now facing obstacles in obtaining fertile and productive land. Unfertile land can be improved by applying biofertilizers. The biofertilizer that can be utilized is Arbuscular Mycorrhizal Fungi (AMF). This research effort was conducted by testing fertilizers that form the best combination for the propagation of AMF spores. This study aims to test and determine the combination of Glomus sp. Spores and several types of fertilizers can optimize the increase in spore quantity and identify the best fertilizer for enhancing colonization on maize roots (Zea mays L.). The research used a Randomized Block Design with four treatments on corn plants over 60 days, namely a positive control using 10 Glomus sp. spores without fertilizer combination (A); 10 Glomus sp. spores and 5 g of NK fertilizer (B); 10 Glomus sp. spores and 20 mL of Johnson's nutrient solution (C); and 10 Glomus sp. spores and 10 g of compost fertilizer (D). The observed parameters are root length; wet and dry weight of the roots; wet and dry weight of the shoots; the number of spores for each treatment; and the percentage of AMF colonization on the roots. Spores were obtained through the wet sieving method. The colonization calculation was performed using the slide method. Quantitative data analysis using ANOVA followed by Duncan's test. The research results show that combining fertilizer with Glomus sp. spores can optimize colonization and the number of mycorrhizal spores in the soil. The best degree of the host plant’s root colonization is shown in combination with NK fertilizer, which is 84.56%. (very high).
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