Skrining Isolat Bakteri Pelarut Kalium Melalui Uji Hipersensitivitas, Uji Hemolisis, Serta Pengujian Viabilitas
DOI:
https://doi.org/10.24843/Keywords:
hypersensitivity, hemolysis, viability, solubilizing potassium bacteriaAbstract
Screening of Potassium Solubilizing Bacteria Using Hypersensitivity, Hemolysis, and Viability Tests. This study aimed to evaluate the biosafety and viability of Potassium Solubilizing Bacteria (KSB) as potential biofertilizers through hypersensitivity testing on tobacco plants, hemolysis testing on Blood Agar media, and viability assessment using molasses and zeolite as carrier media stored at 27oC (room temperature) and 4oC (refrigerator). Hypersensitivity testing showed that the KSB isolate was non-pathogenic to plants, as indicated by negative reactions on tobacco leaves. Hemolysis testing indicated gamma-hemolysis, with no clear zones or color changes on Blood Agar, confirming that the isolate is non-pathogenic and safe for humans and animals. Viability was assessed using Total Plate Count (TPC) on molasses and zeolite as carrier media at 27 oC and 4 oC on days 14 and 28 after inoculation. The results showed that the KSB isolate in molasses stored at 27°C exhibited the highest population growth, reaching 5,6x10⁸ CFU/ml on day 14 and 6,7x10⁹ CFU/ml on day 28. In comparison, the KSB isolate in zeolite at the same temperature showed lower growth, 3,9x10⁸ CFU/g on day 14 and 1,1x10⁹ CFU/g on day 28. Both carriers stored at 4 °C showed slight declines in population, reaching 7,7x105 CFU/ml (molasses) and 3,9x106 CFU/g (zeolite) on day 28. These results indicate that molasses at room temperature (27 °C) is the most suitable carrier for maintaining viable KSB populations, supporting the development of safe and effective potassium-solubilizing biofertilizers that improve plant nutrient availability and ensure biosafety.
References
Amaria, W., Sinaga, M. S., Mutaqin, K. H., Supriadi, & Widodo. (2023). Hemolysis and hypersensitives test ease culture collection management of antagonistic bacteria. J. Trop. Plant Pest Dis, 23(2), 24–30.
Azeem, M., Hassan, T. U., Tahir, M. I., Ali, A., Jeyasundar, P. G. S. A., Hussain, Q., Bashir, S., Mehmood, S., & Zhang, Z. (2021). Tea leaves biochar as a carrier of Bacillus cereus improves the soil function and crop productivity. Appl. Soil Ecol., 157, 103732.
Bhardwaj, S., Huda, S., Jayasena, V., Ahmed, T., & Chen, Z. H. (2023). Potassium transport and use efficiency for sustainable fertigation in protected cropping. Journal of Sustainable Agriculture and Environment, 2(3), 346–356.
Buxton, R. (2005). Blood agar plates and hemolysis protocols. Washington: American Society for Microbiology Pr.
Devianto, L. A., Latunussa, C. E. L., Helmy, Q., & Kardena, E. (2020). Biosurfactants production using glucose and molasses as carbon by Azotobacter vinelandii and soil washing application in hydrocarbon-contaminated soil. IOP Conf. Series: Earth and Environmental Science, 475, 1–6.
Doni, S., Gispert, M., Perruzzi, E., Macci, C., Mattii, G. B., Manzi, D., Masini, M. C., & Grazia, M. (2020). Impact of natural zeolite on chemical and biochemical properties of vineyard soils. Soil Use Manag, 37(4), 832–842.
Etesami, H., Emami, S., & Alikhani, H. A. (2017). Potassium solubilizing bacteria (KSB): Mechanisms, promotion of plant growth, and future prospects a review. Journal of Soil Science and Plant Nutrition, 17(4), 897–911. https://dx.doi.org/10.4067/S0718-95162017000400005.
Hendriana, A., Wiyoto, & Lesmanawati, W. (2020). Penambahan konsentrasi molase berbeda untuk perbaikan kualitas air dan produksi udang vaname (Litopeneus vannamei) (BOONE 1931). Jurnal Sains Terapan, 10(1), 60–68.
Herdiyantoro, D., Setiawati, M.R., & Simarmata, T. (2022). Reaksi hipersensitif daun tembakau oleh isolat bakteri pelarut kalium pada praformulasi pupuk hayati. Soilrens, 20(2), 72–77.
Karmakar, K., Patil, S. S., & Kundu, B. (2024). From soil to oil: The crucial roles of potassium and sulphur in enhancing oilseed crop quality. Journal of Advances in Biology & Biotechnology, 27(8), 335–346.
Kementerian Pertanian Republik Indonesia. (2019). Peraturan Menteri Pertanian Republik Indonesia Nomor 1 Tahun 2019 tentang Pupuk Organik, Pupuk Hayati, dan Pembenah Tanah. Jakarta: Kementerian Pertanian.
Khalisha, A., Widyastuti, R., & Chaniago, I., A. (2022). Use of phosphorus- and potassium-solubilizing multifunctional microbes to support maize growth and yield. Sains Tanah-Journal of Soil Science and Agroclimatology, 19(1), 91–98.
Madaras, M., & Koubová, M. (2015). Potassium availability and soil extraction tests in agricultural soils with low exchangeable potassium content. Plant Soil Environment, 61(5), 234–239.
Manning, D.A.C. (2010). Mineral sources of potassium for plant nutrition. A review. Agronomy for Sustainable Development, 30, 281–294.
Mellyanawaty, M., Purnomo, C. W., & Budhijanto, W. (2017). Pengaruh penambahan zeolit alam termodifikasi sebagai media imobilisasi bakteri terhadap dekomposisi material organik secara anaerob. Jurnal Rekayasa Proses, 11(1), 36–42.
Pudjiwati, E. H., & Hamid, N. B. (2020). Viabilitas dan aktivitas bakteri pelarut fosfat indigenus pada beberapa bahan pembawa cair. Jurnal Boreneo Saintek, 3(2), 85-92.
Putri, S. M., Anas, I., Hazra, F., Citraresmini, A. (2010). Viabilitas inokulan dalam bahan pembawa gambut, kompos, arang batok dan zeolit yang disteril dengan iradiasi sinar gamma Co-60 dan mesin berkas elektron. Jurnal Tanah dan Lingkungan, 12(1), 23–30.
Raju, K., Thirupathi, S. M., Khader, S. A. R. M. A., Pugalenthi, S., Kumar, S. K., Vel, B. S., Sivamalar, S. S. M., & Marichamy, S. (2025). Assessment of organic and inorganic carrier material based biofertilizers: A review. Curr Agri Res, 13(2), 453–456.
Rani, K., Datta, A., Jat, H. S., Choudhary, M., Sharma, P. C., & Jat, M. L. (2023). Assessing the availability of potassium and its quantity-intensity relations under long term conservation agriculture based cereal systems in North-West India. Soil and Tillage Research, 228, 105644.
Rani, V., & Sengar, R. S. (2020). Use of potassium bio-fertilizers technology for sustainable agriculture in dry areas. Journal of Pharmacognosy and Phytochemistry, 9(6), 1944–1946.
Subhash, S., Singh, S., & Prajapati, S. (2022). Potassium and its forms in soil. The Agriculture Magazine, 1, 10–13.
Suroto, A., Mugiastuti, E., Tarjoko, Oktviani, E., & Bahrudin, M. (2023). Uji hipersensitivitas bakteri terbawa serangga pada lahan penanaman cabai di Kabupaten Banyumas. Gontor Agrotech Science Journal, 9(1), 67–81.
Syarifain, R.I., Rashaun, M.R., Anggrainy, E.D., & Simarmata, R. (2022). Formulasi pembawa rizobakteri penambat nitrogen dan pelarut fosfat, serta aplikasinya di pembibitan kelapa sawit yang diberi komposisi dan dosis amelioran yang berbeda. Jurnal Kultivasi, 21(2), 190–201.
Tränkner, M., Tavakol, E., & Jákli, B. (2018). Functioning of potassium and magnesium in photosynthesis, photosynthate translocation and photoprotection. Physiologia Plantarum, 163, 414–431.
Vassileva, M., Mocali, S., Canfora, L., Malusà, E., Moral, L. F. G. D., Martos, V., Flor-Peregrin, E., & Vassilev, N. (2022). Safety level of microorganism-bearing products applied in soil-plant systems. Front. Plant Sci, 13, 862875.
Wartono, Suryadi, Y., & Susilowati, D. N. (2012). Keefektifan formulasi bakteri Burkholderia cepacian isolat E76 terhadap Rhizoctonia solani Kühn pada pertumbuhan tanaman padi di laboratorium. Jurnal Agrotropika, 17(2), 39–42.
Yasin, M., Munir, I., & Faisal, M. (2016). Can Bacillus spp. enhance K+ uptake in crop species. In Potassium Solubilizing Microorganisms for Sustainable Agriculture. Springer, New Delhi.
