Detection and Molecular Identification of Candidatus Liberibacter asiaticus in Mandarin Citrus (Citrus reticulata), a Local Cultivar from Tejakula, Bali, Indonesia, Based on 16S rRNA Gene Analysis
DOI:
https://doi.org/10.24843/Keywords:
Candidatus Liberibacter asiaticus, CVPD, mandarin citrus, Tejakula, 16S rRNAAbstract
Citrus Vein Phloem Degeneration (CVPD), also known as Huanglongbing (HLB), has become one of the main challenges in citrus production in Indonesia, including mandarin citrus var. Tejakula from Bali. The disease is associated with Candidatus Liberibacter asiaticus (CLas), a bacterium that inhabits phloem tissue and cannot be maintained in artificial culture, making molecular approaches essential for its detection. This study aimed to confirm the presence of CLas in mandarin citrus var. Tejakula in Bali using 16S rRNA gene analysis. Leaf samples showing CVPD-like symptoms were collected from four different locations and subjected to DNA extraction. Detection was performed using PCR with specific primers, followed by electrophoresis and sequence analysis. The results indicated that two samples, originating from Pecatu and Tukad Sumaga, were positively detected. Sequence comparison revealed a high level of similarity with reference sequences available in GenBank, confirming the identity of the pathogen. These findings provide molecular evidence of CLas infection in Bali and demonstrate the reliability of 16S rRNA-based detection for CVPD diagnosis.
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